Research technician | Molecular Cell Biology and Immunology
The project Keloid disorder is an invalidating benign scar tumor of the skin. In predisposed patients after a small inflammation or surgery, especially on the thorax, shoulders, ears or jawline, excessive fibrosis can develop. These raised, red, itchy and painful fibrotic skin tumors diminish the quality of life of the affected patient. Unfortunately, a good therapy is lacking and surgery with a high recurrence rate of more than 90%, makes it even worse. Although it is known that changes in immune response following trauma can lead to keloid formation, better insight in the exact inflammatory balances during healing would be of utmost importance to develop a better treatment strategy. Within this project we aim to develop primary in vitro assays to test molecules for their ability to skew differentiation of monocytes or macrophages. In addition, we want to study keloids in more detail using single cell transcriptomics in order to find targets to regulate inflammation and fibrosis within keloids.
As research technician you will be expected to perform the following tasks:
isolation and in vitro culture of primary monocytes and differentiation into different types of macrophages
test the effect of molecules on differentiation of monocytes/macrophages
phenotypic and functional analysis of different types of macrophages by flow cytometry and real time metabolism measurements
set up protocol for isolation of single cells or nuclei from surgically resected keloids for single cell transcriptomics
We are looking for a highly talented, enthusiastic, and exceptionally motivated research technician:
With a training in biomedical sciences (HLO or equivalent) with affinity for immunology and/or molecular biology.
With experience in cell culture and preferably flow cytometry, metabolic analysis and single cell technologies
A keen interest in translational research
Due to the interdisciplinary nature of this project and collaboration with industry partners, we seek a strong team player with excellent communicative skills. The ability to work within an international scientific research group is essential.
The monthly wage will increase yearly to the maximum salary in the fourth year of 3,196 € gross per month.
Additional benefits include an 8.3% end-of-year bonus and 8% holiday pay.
Your pension premium will be paid by us for 2/3.
We reimburse 75% of your OV travel costs (commuting costs).
For Dutch citizens it is mandatory to provide a VOG (a declaration of good behaviour).
The position is for a period of 1 year. After a good evaluation, and depending on available finance, your contract can be extended with an additional period of max. 3 years.
This research is embedded in the Amsterdam infection and immunity research (VUmc). This institute facilitates translation of discoveries in immunology into clinical applications with patient benefit.
You will be working within the Skinlab of Prof. Gibbs, under the direct supervision of Dr Koning, in the department of Molecular Cell Biology and Immunology (MCBI) of the Amsterdam UMC, located in the O|2 building in Amsterdam. Within Skinlab we aim to develop state of the art in vitro models for skin research including organ on chip models.
MCBI hosts the state of the art Flow-Cytometry and Microscopy facilities and provides the relevant education such as Advanced Flow Cytometry and Microscopy courses. The research tasks are closely embedded in ongoing research activities of the participating departments.
Your application must include: a one-page personal motivation letter, a CV including the names and contact details of two recent references. Only complete applications will be considered.
For more information about this position, you can contact dr. Jasper Koning, via email firstname.lastname@example.org.
For more information about the application procedure, you can contact Brend de Jong corporate recruiter, via telephone number: +31 (0)20 444 8704.
You can apply till 17 September 2021 by using the apply button.