Job Properties
  • Job Type
    Full-time Position
  • Category
    Research & Science
  • Languages
    English
  • Experience Required
    Entry
  • Degree Required
    Bachelor
    • Province
      Amsterdam
    • Date Posted
      November 24,2021
    • JSS
    • VISA
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    • Career Consultation
    • COVERLETTER CHECK
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    PhD Endothelial Integrity

    About the role

    We are looking for a motivated PhD student who has been trained in cell culture and molecular techniques (preferred background in (molecular) biology, biochemistry or cell biology), who is willing to join our research team to work on this project. You will use primary human endothelial cells in combination with biochemical and cell biological techniques, including (super resolution) live-cell confocal microscopy.

    What you are going to do

    Within the department of Physiology at Amsterdam UMC, location VUmc, we focus on basic and translational research in the context of cardiovascular disease. In our group, we are particularly interested in the molecular mechanisms that control cell-cell contact between human endothelial cells. These cells line all our blood vessels and are criticial ‘gatekeepers’ that control coagulation and the transport of fluids and immune cells from the circulation to the tissues and vice versa. This is important during inflammation, for example, and since inflammation is at the basis of cardiovascular disease, understanding its underlying mechanisms is key to the future development of effective therapies.

    Profile

    What we expect from you

    Prerequisites are as follows:

    • Master of Science degree in Biology, Biomedical Sciences or equivalent;
    • significant experience with cell biological and biochemical= techniques;
    • a creative and flexible work attitude;
    • a team player with excellent communication skills;
    • the capacity to work independently.

    Our offer

    We offer you ample opportunity for development, deepening and broadening, additional training and a place to grow! Working at AUMC means working in an inspiring and professional environment where development is encouraged in every respect.

    • We offer a four year (PhD) contract starting for 12 months, to be extended for another 36 months upon evaluation.
    • We provide further training in a PhD educational program and create possibilities for career development.
    • In addition to a good basic salary, we offer 8.3% end-of-year bonus and 8% holiday allowance.
    • Your pension is accrued with the ABP; VUmc contributes a large part of the premium (70%).
    • In addition to excellent accessibility by public transport, we reimburse 75% of your OV travel costs (commuting costs).
    • For an overview of all our other terms of employment, see https://www.werkenbijvumc.nl/watwijbieden/

    About the project

    The regulation of cell-cell contact in endothelium is a major research topic in the group. Since this cell-cell contact requires the cytoskeleton, we focus on a group of proteins, the Rho-like small GTPases, that are central regulators of the actin cytoskeleton. Recently, we identified GTPases that are positive or negative modulators of endothelial cell-cell contact. We also studied the regulation of key Rho GTPases by post-translational modification, in particular ubiquitination.

    In this project, we aim to study Rho GTPase regulation and modification 2D and 3D models for the microcirculation. We will use human primary-, or iPSC-derived endothelial cells in the absence or presence of flow and in resting or activated (i.e inflammation) conditions. Gain and loss of function studies as well as manipulation of signaling pathways in combination with live cell confocal imaging will be used to generate new fundamental insights on the control of endothelial integrity.

    About the workplace

    Where you are going to work

    You will work in a team of PhD students and postdocs that all study various aspects of endothelial and vascular biology, such as metabolic disorders, obesity-related vascular dysfunction and aging. In addition, the department has strong research lines in the fields of cardiomyocyte contractility and studies the crosstalk between heart muscle cells and the endothelium. The dept of Physiology is part of the Amsterdam UMC Research Center ‘Amsterdam Cardiovascular Sciences and of the AUMC ‘hartcentrum’. This means that the project is well embedded in department as well as in the larger AUMC research institute.

    Let's meet

    For more information about this position, you can contact Peter Hordijk, p.hordijk@amsterdamumc.nl

    For more information about the application procedure please contact Tanja Hart, Recruitment adviser, via t.hart@amsterdamumc.nl or via 06-21603178.

    A reference check, screening and hiring test may be part of the procedure. Read hier whether this also applies to you.

    For Dutch citizens it is mandatory to provide a VOG (Verklaring Omtrent Gedrag). Foreign citizens need to provide comparable police clearing certificates.

     
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